p jak2  (Cell Signaling Technology Inc)

Journal: Molecular Medicine Reports

Article Title: Naringin ameliorates intestinal injury in ulcerative colitis model mice by modulating the JAK2/STAT3 signaling pathway

doi: 10.3892/mmr.2026.13805

Figure Lengend Snippet: Naringin inhibits JAK2/STAT3 signaling and restores tight junction proteins in dextran sulfate sodium-induced colitis. (A) Representative western blotting images of p-JAK2, JAK2, p-STAT3, STAT3, IL-6, occludin, ZO-1 and β-actin expression in colon tissues. Densitometric semi-quantification of the relative protein expression levels of (B) p-JAK2/β-actin, (C) JAK2/β-actin, (D) p-JAK2/JAK2, (E) p-STAT3/β-actin, (F) STAT3/β-actin, (G) p-STAT3/STAT3, (H) IL-6/β-actin, (I) occludin/β-actin and (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. control group; # P<0.05 and ## P<0.01 vs. normal group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; C, control group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; IL-6, ZO-1, zona occludens-1.

Article Snippet: Membranes were subsequently incubated separately with primary antibodies for p-JAK2 (1:1,000; cat. no. bs-2485R; BIOSS), JAK2 (1:1,000; cat. no. bs-0908R; BIOSS), p-STAT3 (1:1,000; cat. no. bs-1658R; BIOSS), STAT3 (1:1,000; cat. no. bs-55208R; BIOSS), occludin (1:1,000; cat. no. A2601; ABclonal Biotech Co., Ltd.), ZO-1 (1:1,000; cat. no. A0659; ABclonal Biotech Co., Ltd.) and β-actin (1:1,000; cat. no. ab8227; Abcam), and then with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:5,000; cat. no. ab6721; Abcam).

Techniques: Western Blot, Expressing, Control

Journal: Molecular Medicine Reports

Article Title: Naringin ameliorates intestinal injury in ulcerative colitis model mice by modulating the JAK2/STAT3 signaling pathway

doi: 10.3892/mmr.2026.13805

Figure Lengend Snippet: Naringin suppresses JAK2/STAT3 activation in IL-6-stimulated Caco-2 cells with STAT3 silencing. (A) Western blot analysis of p-JAK2, JAK2, p-STAT3, STAT3, occludin and ZO-1 in Caco-2 cells under indicated treatments. (B) Validation of STAT3 knockdown efficiency: Relative STAT3 expression in cells transfected with siSTAT3 vs. siNC. ## P<0.01 vs. siNC. Densitometric semi-quantification of relative protein expression levels of (C) p-JAK2/β-actin, (D) JAK2/β-actin, (E) p-JAK2/JAK2, (F) p-STAT3/β-actin, (G) STAT3/β-actin, (H) p-STAT3/ STAT3, (I) occludin/β-actin, (J) ZO-1/β-actin. Data are presented as mean ± SEM. n=3. *P<0.05 and **P<0.01 vs. IL-6 group; ## P<0.01 vs. normal group; Δ P<0.05 and ΔΔ P<0.01 IL-6 + Nar group vs. IL-6 + Nar + siSTAT3 group. One-way ANOVA followed by Tukey's post-hoc test was applied. N, normal group; Nar, naringin group; Mes, mesalazine group; p-, phosphorylated; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; ZO-1, zona occludens-1; si, small interfering RNA; NC, negative control.

Article Snippet: Membranes were subsequently incubated separately with primary antibodies for p-JAK2 (1:1,000; cat. no. bs-2485R; BIOSS), JAK2 (1:1,000; cat. no. bs-0908R; BIOSS), p-STAT3 (1:1,000; cat. no. bs-1658R; BIOSS), STAT3 (1:1,000; cat. no. bs-55208R; BIOSS), occludin (1:1,000; cat. no. A2601; ABclonal Biotech Co., Ltd.), ZO-1 (1:1,000; cat. no. A0659; ABclonal Biotech Co., Ltd.) and β-actin (1:1,000; cat. no. ab8227; Abcam), and then with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:5,000; cat. no. ab6721; Abcam).

Techniques: Activation Assay, Western Blot, Biomarker Discovery, Knockdown, Expressing, Transfection, Small Interfering RNA, Negative Control

Journal: Frontiers in Cellular Neuroscience

Article Title: (Ba,Ca)(Ti,Sn)O 3 -based piezoelectric ceramics promotes neuroprotection by regulating microglial IL-6/JAK2/STAT3 signaling pathway

doi: 10.3389/fncel.2026.1793008

Figure Lengend Snippet: BCTS-CM attenuates activation of the IL-6/JAK2/STAT3 pathway in PC12 cells. (A) Western blot results of IL-6/JAK2/STAT3 signaling pathway. (B,C) Statistical analysis of P-JAK2/JAK2 and P-STAT3/STAT3 expression. Data are expressed as mean ± SEM, n = 5 vs. Con group, a P ≤ 0.01 vs. LPS group, b P ≤ 0.05 vs. BCTS-CM group, c P ≤ 0.05.

Article Snippet: JSM-5900 Scanning Electron Microscope (Japan), Xpert-PRO X-ray Diffractometer (Netherlands), Water Contact Angle Goniometer JC2000D1 (Shanghai Zhongchen Digital Technology Equipment Co., Ltd., China), Quasi-static d 33 Meter ZJ-3AN (Institute of Acoustics, Chinese Academy of Sciences, China), Ultrasound Stimulator (GV-CSI1.0) (Green Valley BrainTech (ShenZhen) Medical Technology Co., Ltd. (China), BV2, PC12 (Wuhan Pricella Biotechnology Co., Ltd., China), Rabbit Anti-iNOS (Thermo Fisher Scientific, Inc.), Rabbit Anti-Iba-1, Arg-1, NeuN, Goat Anti-Rabbit IgG H&L (Alexa Fluor 488), Goat Anti-Mouse IgG H&L (Alexa Fluor 594) (Abcam, UK), Rabbit Anti-CD86, CD206, β-actin, HRP-conjugated Goat Anti-Rabbit IgG (H + L) (Wuhan Proteintech Biotechnology Co., Ltd., China), Rabbit Anti-IL-6, TNF-α, IL-4, IL-10, TGF-β1 (ImmunoWay, United States), Rabbit Anti-IL-1β, JAK2, P-JAK2, STAT3, P-STAT3, BAX, Bcl-2, Cleaved Caspase-3 (Shenyang Wanlei Biotechnology Co., Ltd., China), BCA Protein Assay Kit, CCK8 Cell Viability Assay Kit, LDH Cytotoxicity Assay Kit, SOD Activity Assay Kit, CAT Activity Assay Kit, GSH-Px Activity Assay Kit, MDA Assay Kit, Dapi (Beijing Solarbio Science & Technology Co., Ltd., China), LPS (Sigma, Germany), IL-6, AG490 (MCE, Germany), Laser Confocal Microscope (Olympus Corporation, Japan), SDS-PAGE Electrophoresis System (BIO-RAD, USA).

Techniques: Activation Assay, Western Blot, Expressing

Journal: Frontiers in Cellular Neuroscience

Article Title: (Ba,Ca)(Ti,Sn)O 3 -based piezoelectric ceramics promotes neuroprotection by regulating microglial IL-6/JAK2/STAT3 signaling pathway

doi: 10.3389/fncel.2026.1793008

Figure Lengend Snippet: BCTS attenuates the expression of the IL-6/JAK2/STAT3 signaling pathway . (A) Western blot results of IL-6/JAK2/STAT3 signaling pathway. (B–D) Statistical analysis of IL-6, P-JAK2/JAK2 and P-STAT3/STAT3 expression. Data are expressed as mean ± SEM, n = 5 vs. Sham group, a P < 0.05, b P < 0.01 vs. SCI group, c P < 0.05.

Article Snippet: JSM-5900 Scanning Electron Microscope (Japan), Xpert-PRO X-ray Diffractometer (Netherlands), Water Contact Angle Goniometer JC2000D1 (Shanghai Zhongchen Digital Technology Equipment Co., Ltd., China), Quasi-static d 33 Meter ZJ-3AN (Institute of Acoustics, Chinese Academy of Sciences, China), Ultrasound Stimulator (GV-CSI1.0) (Green Valley BrainTech (ShenZhen) Medical Technology Co., Ltd. (China), BV2, PC12 (Wuhan Pricella Biotechnology Co., Ltd., China), Rabbit Anti-iNOS (Thermo Fisher Scientific, Inc.), Rabbit Anti-Iba-1, Arg-1, NeuN, Goat Anti-Rabbit IgG H&L (Alexa Fluor 488), Goat Anti-Mouse IgG H&L (Alexa Fluor 594) (Abcam, UK), Rabbit Anti-CD86, CD206, β-actin, HRP-conjugated Goat Anti-Rabbit IgG (H + L) (Wuhan Proteintech Biotechnology Co., Ltd., China), Rabbit Anti-IL-6, TNF-α, IL-4, IL-10, TGF-β1 (ImmunoWay, United States), Rabbit Anti-IL-1β, JAK2, P-JAK2, STAT3, P-STAT3, BAX, Bcl-2, Cleaved Caspase-3 (Shenyang Wanlei Biotechnology Co., Ltd., China), BCA Protein Assay Kit, CCK8 Cell Viability Assay Kit, LDH Cytotoxicity Assay Kit, SOD Activity Assay Kit, CAT Activity Assay Kit, GSH-Px Activity Assay Kit, MDA Assay Kit, Dapi (Beijing Solarbio Science & Technology Co., Ltd., China), LPS (Sigma, Germany), IL-6, AG490 (MCE, Germany), Laser Confocal Microscope (Olympus Corporation, Japan), SDS-PAGE Electrophoresis System (BIO-RAD, USA).

Techniques: Expressing, Western Blot

Journal: Bioresources and Bioprocessing

Article Title: Propionate-engineered probiotics reduce radiation-induced intestinal damage

doi: 10.1186/s40643-026-01020-9

Figure Lengend Snippet: Engineered probiotic PG mitigated radiation-induced intestinal injury by regulating the SOCS1/JAK2/STAT3 pathway. a Principal Component Analysis (PCA) revealed differences in gene expression levels among groups. b KEGG Enrichment BarPlot for IR vs. IR + PG displayed the number of differential genes in the pathway represented on the horizontal axis, pathway names on the vertical axis, and colors representing KEGG Level 1 classification. c KEGG-enriched bar graph displaying the top 20 KEGG pathways with the smallest P-values. d The results of the KEGG enrichment analysis werw presented as a scatter plot. (e) The heatmap illustrated the relative expression levels of JAK-STAT signaling pathway-related genes in the IR and IR + PG groups. f Western blot analysis demonstrated the protein expression levels of SOCS1, p-JAK2, JAK2, p-STAT3, and STAT3 across groups

Article Snippet: The primary antibodies included anti-SOCS1 (PSH09-68), JAK2 (SY0245), p-JAK2 (SY24-03), STAT3 (SY24-08), p-STAT3 (SZ43-01), and GAPDH (SA30-01), all purchased from HuaBio-Antibodies Co., Ltd (Hangzhou, China).

Techniques: Gene Expression, Expressing, Western Blot